The Toronto Blue Jays first baseman was pulled in the bottom of the fourth inning against the Washington Nationals with right hamstring tightness, the team announced.
Guerrero appeared to suffer the injury when running the bases in the top of the third inning.
After hitting a groundball to third base, Guerrero was able to advance to second on a throwing error by Jorbit Vivas. When he rounded first, Guerrero looked to have some discomfort on his face as he made his way to second.
He stayed in the game for the remainder of the inning and also came back out on defence for the bottom of the third. He was replaced by Kazuma Okamoto at first base in the bottom of the fourth.
Guerrero was 1-for-2 at the plate before being forced to exit.