The Burnaby, B.C., native appeared to run around the bases gingerly in the top half of the fourth inning before being removed from the game in the bottom half.
Dylan Beavers moved from centre field to right, and Colton Cowser entered the game to replace O’Neill and play centre.
O’Neill, 31, is having a second straight down season, slashing .205/.297/.360 with six home runs and 15 RBIs. He hit 31 home runs for the Red Sox in 2024.
The Red Sox tied their franchise record with a 15th straight win in Game 1 of a split doubleheader against the Orioles on Wednesday and have the chance to break it in Game 2.